MALDI/ESI Injector
From Spectroglyph, LLC
Contents
MALDI/ESI Injector
Instrumentation
Spectroglyph LLC offers a new product, MALDI/ESI Injector interface, concurrently enabling both elevated pressure (EP) MALDI and ESI capabilities.
The interface is compatible with Thermo's OrbitrapTM Q Exactive Classic, Q Exactive Focus, Q Exactive Plus, Q Exactive HF/HFX and OrbitrapTM Elite, OrbitrapTM Velos and OrbitrapTM XL, as illustrated in Figures 1-4.
The interface is compatible with Thermo's OrbitrapTM Q Exactive Classic, Q Exactive Focus, Q Exactive Plus, Q Exactive HF/HFX and OrbitrapTM Elite, OrbitrapTM Velos and OrbitrapTM XL, as illustrated in Figures 1-4.
The MALDI/ESI Injector layout is shown in detail in Figure 5.
Stage movement is synchronized with Orbitrap spectrum acquisition using hardware triggers.
As a result, microscan numbers reported in a position file (XML format) are directly correlated to spectrum numbers in Thermo's RAW file.
The latter is processed by Xcalibur software (Thermo Fisher Scientific).
Hardware-level synchronization makes image reconstruction and alignment readily available.
As a result, microscan numbers reported in a position file (XML format) are directly correlated to spectrum numbers in Thermo's RAW file.
The latter is processed by Xcalibur software (Thermo Fisher Scientific).
Hardware-level synchronization makes image reconstruction and alignment readily available.
Imaging Capability
A dual translation stage used in MALDI/ESI Injector (Zaber, Vancouver, BC) handles standard hystology slides, whose dimensions are 75 mm x 25 mm, providing for a large area of sampling at a resolution of 50 nm/step. To enable efficient operation of an Orbitrap mass spectrometer, MALDI/ESI Injector is equipped with a computer-controlled pneumatic gate valve, which allows for rapid exchange of sample slides while keeping pressure of 2x10-10 mbar in the analyzer.
Shown in Figure 6, a UV laser beam is routinely focused to a spot of 20 µm in diameter. In MALDI mode, a diode-pumped solid state YAG laser is employed. Laser parameters are the following: single mode, an output energy of 20 µJ, 2 mrad beam divergence, 1 mm beam diameter at laser aperture, a wavelength of 355 nm, a user-defined repetition rate of 200 Hz to 2 kHz.
Shown in Figure 6, a UV laser beam is routinely focused to a spot of 20 µm in diameter. In MALDI mode, a diode-pumped solid state YAG laser is employed. Laser parameters are the following: single mode, an output energy of 20 µJ, 2 mrad beam divergence, 1 mm beam diameter at laser aperture, a wavelength of 355 nm, a user-defined repetition rate of 200 Hz to 2 kHz.
Lipids
Figure 7 shows signals obtained with a rat brain tissue sample. Experimental setting: negative ion mode, 60,000 mass resolution, 30 µm spatial resolution, acquisition rate of 7.2 pixels/s. 147,508 pixels, 349 nm laser wavelength, 2 µJ laser energy.
Images at the bottom panel of Figure 7 demonstrate spatial distributions of the two closely spaced in m/z domain (δm = 50 mmu) lipid molecules.
Figure 8 shows data obtained with a mouse kidney tissue at 100 µm step size, 120,000 mass resolution (256 ms transient), 32767 pixels, and an acquisition rate of 3.2 pixels/s.
Images at the bottom panel of Figure 7 demonstrate spatial distributions of the two closely spaced in m/z domain (δm = 50 mmu) lipid molecules.
Figure 8 shows data obtained with a mouse kidney tissue at 100 µm step size, 120,000 mass resolution (256 ms transient), 32767 pixels, and an acquisition rate of 3.2 pixels/s.
Peptides and Proteins
Spectroglyph provides a complete unsurpassed solution for EP MALDI imaging with an OrbitrapTM Mass Spectrometer at high spatial and mass resolution, < 20 µm and 240,000, respectively.
Figure 10 shows signals of 1 pmol Bovine Serum Albumin (BSA) tryptic digest obtained with a Q Exactive Mass Spectrometer.
Figure 10 shows signals of 1 pmol Bovine Serum Albumin (BSA) tryptic digest obtained with a Q Exactive Mass Spectrometer.
Figure 11 shows EP MALDI signals of 1 pmol intact protein samples. Upper panel - insulin chain B, bottom panel - thymosin β4.
Contact Us
Due to proven performance enhancing capabilities, MALDI/ESI Injector has already received attention in the imaging field. Interested customers and distributors can contact us for a quotation by writing to info@spectroglyph.com