From Spectroglyph, LLC
Our Smart MS2 software suite is used to efficiently control hybrid ion trap FTMS instruments manufactured by Thermo Fisher Scientific. The objective is to provide reliable identifications of peptides and proteins from highly complex biochemical mixtures. In real time, the software fully controls instrumental settings and data acquisition parameters. To enable highly efficient identification strategy, our algorithm selects chromatographic profiles of potential precursor candidates and initiates their collisional activation at the maximum of precursor signals in chromatographic domain. Fragment ion signal from consecutive LC runs aligned relative to each other to ensure minimum overlap in identifications from one run to another.
The software is amenable to user-defined modifications for advanced data activation strategies, and can be used as a platform for development of novel proteomic approaches with hybrid FTMS instruments.
Using our proprietary alignment algorithms, unique precursor candidates are selected from one run to the next with <10% overlap. This ensures deeper analysis of proteome per unit time.
with Exclusion and Alignment Peptide Lists.
experiments with Smart MS2 approach.